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The test quality criteria were fulfilled.

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Results with reference substance positive control : - Results with reference substance valid? YesThe value EC50 for inhibition of growth rate 72h — ErC50 obtained from our last reference test meets the calculated range from the interlaboratory test.

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The sensitivity of test species and proficiency of our laboratory in test performance was proved. Therefore exact value of ErC50 could not be calculated and the value of EC is given in the Dating site uri QC of a range.

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Expert 2. So the nominal concentrations were used for all evaluations and results.

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Executive summary: The test substance, Direct Blue Dating site uri QC, was tested for growth inhibition on algae Desmodesmus subspicatus. The test was performed according to method C. The preliminary test was performed in a range of Dating site uri QC test substance nominal concentrations 1 — mg·L The analytical results showed, that the test substance Direct Blue 85 was sufficiently stable in the test medium at conditions of the test in concentration range 1 — mg·L The highest inhibition of growth rate was Based on no toxicity of the test substance found in the preliminary test measured by growth ratethe limit test was performed subsequently.

Only the value of 72 hour — ErC50 is needed for the classification and risk assessment of chemicals, so the value of 72 hour — EyC50 was not calculated.

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The concentration of mg·L-1 was tested in the limit test. The inhibition of growth rate was The guideline specify that if evidence is available to demonstrate that the concentration of the test substance in limit test has been satisfactorily maintained within ± 20 per cent of the nominal or measured initial concentration throughout the limit test, then the results can be based on nominal or measured initial values, which is the case of this study.

The nominal concentrations were used for all evaluations and results.

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